Gephi forums

Hi, I've just started using Gephi to look at gene regulatory networks and have run into the problem of showing up and down regulation. It seems easiest to simply connect two nodes with a red arrow to show down regulation and a green one to show up regulation, though I can't figure out how to assign specific colors to specific edges. I would quite appreciate if you would help me out. Thanks!

Hello,

In Gephi the Partition module is done for that, if you have a attribute for each edge, 'DOWN' or 'UP' in a 'regulation' column, the Partition will propose you to color with this attribute.

Thank you for getting back to me so quickly. Would you mind being more detailed if possible? I'm a bit confused about creating the new column.

Sure, in which format do you input your data ?

I've been using excel spreadsheets saved as ".csv" where one column is a list of genes and the one directly next to it is another list of genes. I tried to add "up" and down" next to each row of two genes but that only resulted in more nodes.

I see, the CSV import can't import additional data. I would recommend to go to the GDF format, which is close from CSV.

Normally in GDF you define nodes and edges, but you can ignore nodes they wil be automatically created. However you need to provide the node line header. Therefore your file will be something like this:
Then in Gephi go to the Partition module and select "Edges". Click on the green button to refresh the columns and you should see 'regulation' there.

Cool, I'll give this a shot. Thanks again for the advice.